Implemented kill switches: 2
Kill Switch 1
Link
http://2014.igem.org/Team:Minnesota#Project/slide5
http://2014.igem.org/wiki/images/b/bc/David's_Killswitch_with_Picture_2.pdf
http://www.nature.com/nmeth/journal/v9/n3/pdf/nmeth.1892.pdf
http://2014.igem.org/Team:Minnesota#Project/slide5
http://2014.igem.org/wiki/images/b/b0/Sarah's_Killswitch_with_Pictures_2_%281%29.pdf
Class
Two bacterial strains constantly express exchanged toxin and antitoxin
Toxins
| Name | Number | Comment |
|---|---|---|
| VapC | Kills the cell through RNA cleavage | |
| ParE |
Antagonists
| Name | Number | Comment |
|---|---|---|
| VapB | ||
| ParD |
Topology
Parts
| Name | Number | Comment |
|---|---|---|
| genes for toxin/antitoxin system | ||
| genes for Pilus |
Kill Switch 2
Link
Class
Linearly light induced holin/lysin production
Toxins
| Name | Number | Comment |
|---|---|---|
| Holin | K772101 | Kills the cell by holin system |
| Endolysin |
Antagonists
| Name | Number | Comment |
|---|---|---|
| None |
Topology
Parts
| Name | Number | Comment |
|---|---|---|
| VVD and blue light inducible promoter | Information: http://www.nature.com/nrm/journal/v13/n4/pdf/nrm3307.pdf | |
| gal4 (65) (inactive form of gal4) | ||
| Gal4 inducible promoter |